Could he have hurt himself jumping onto your knee or jumping off? If you are sure he is not hurt it is most likely that he is having a major sulk. Perhaps you could desensitise him to the piccolo - hold it in your hand while he is on your lap, play it when he is in another room etc - perhaps it really hurt his ears last night and maybe he has a sort of ringing in his ears - like you would after a loud rock concert.
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Fiona, Toffee - Heinz Hound, Ojo - Patterdale/JR and Manny - Rough coated Jack Russell
Outside of a dog a book is man's best friend. Inside a dog it's too dark to read!
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